Studies on sulphatases. 4. Arylsulphatase and beta-glucuronidase concentrates from limpets.
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Steroid Sulphatase, Arylsulphatase and ,b-glucuronidase in Marine Invertebrates
The widespread occurrence of sulphatases in the Mollusca was first demonstrated by Soda & Hattori (1933a, b) who showed that these enzymes are mainly concentrated in the digestive glands. That extracts from marine molluscs also possess ,B-glucuronidase activity was first noted by Dodgson, Lewis & Spencer (1952) who studied the optimum conditions for the activity ofthis enzyme and arylsulphatase...
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In the preceding paper (Dodgson & Powell, 1959) it was shown that an arylsulphatase present in the digestive juice and the digestive gland of the edible snail exhibited a shift in optimum pH as the concentration of substrate was increased. This paper describes the partial purification of the digestivegland enzyme and provides further evidence that the shift in optimum pH is not due to the prese...
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Umbelliferone (7-hydroxycoumarin) and its conjugates appear to be metabolites ofcoumarin in the rabbit (Mead, Smith & Williams, 1955). It was observed that, although umbelliferone fluoresced strongly in ultraviolet light at pH 9-10, its conjugates showed little or no fluorescence. The glucuronide and ethereal sulphate of umbelliferone were therefore made and the former was found to be practical...
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1. Rat kidney lysosomal glycoproteins, prelabelled in the N-acetylneuraminic acid and polypeptide portions with N-acetyl[(3)H]mannosamine and [(14)C]lysine, or with N-acetyl-[(14)C]glucosamine, were incubated under various conditions. Autolytic cleavage of labelled N-acetylneuraminic acid and peptide was maximum at pH5.0. 2. N-Acetylneuraminic acid was released more rapidly than peptide during ...
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One hundred and one young-adult female Sprague-Dawley rats were acclimatized to metabolic cages for 2 days. After that time 24-hour urine was collected at a constant cooling temperature of 0-4 degrees C. After gel filtration the enzyme activities were determined, and the resulting values were used to calculate 24-hour excretions. The following reference ranges (2.5 and 97.5 percentiles) were de...
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عنوان ژورنال:
- The Biochemical journal
دوره 55 2 شماره
صفحات -
تاریخ انتشار 1953